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UgtB1 is a riboflavin transporter from Streptococcus thermophilus that belongs to the CzcD family of proton antiporters. Bacteria are unable to synthesize riboflavin from precursor amino acids, and these amino acid biosynthetic steps are closely related to riboflavin biosynthesis. UgtB1 is a member of a family that has also been studied in human pathogens. We have cloned, expressed, purified and characterized UgtB1 from S. thermophilus. We have characterized UgtB1 in vitro by radioactive uptake assays in a variety of conditions, and genetically by biophysical analyses of the functions of UgtB1 mutants. UgtB1 transports riboflavin (Rf) and 7-methyl-8-hydroxy-5-deazaflavin (7HM-8HM). However, significant differences were observed between the results obtained with the two substrates. H(+) gradients enhance UgtB1 activity with Rf as a substrate; differences in protein conformation with low pH are responsible for the depression of Rf transport activity at low H(+) concentrations. UgtB1 does not transport 7HM-8HM. We have also expressed UgtB1 in E. coli and found that UgtB1 can provide enough Rf transport activity to support growth in a riboflavin-limited medium. Altogether, these results suggest that UgtB1 transports Rf as a substrate. This is the first report of an R

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Chennai: The fourteenth of the Indian Premier League (IPL) to be held in Tamil Nadu, the Chennai Super Kings and Rajasthan Royals will be keen to give their respective players their best shot in IPL 5 at the M A Chidambaram Stadium, Chepauk.

There has been a good turnout of foreign players, whose number might have been down in recent years, in these IPLs, with the likes of Cameron White and Paul Collingwood in the Chennai side and Graeme Smith and South Africa’s Faf du Plessis in the Rajasthan side. There have been good highlights and controversies with a number of players coming from the domestic sides of the two franchises being asked to take a back seat, as all their domestic players are included in the national squad of which Australia’s Shane Watson, Marcus Trescothick, Brett Lee and Michael Hussey are already part of.

Among the foreign players in the Rajasthan Royals’ team are two of South Africa’s greatest all-round cricketers, David Miller and Jacques Kallis. Miller is among the 50 greatest cricketers of all time according to the ICC, while Kallis has been to the top of the ICC rankings for over seven years. Trescothick is another great England all-rounder, while Pakistan’s captain Misbah-ul-Haq, Sri Lanka’s Kusal Mendis, Sri Lanka’s Lasith Malinga, Misbah